Molecular detection of Chlamydia trachomatis infection among males with abnormal semen
Many studies revealed that Chlamydia trachomatis is the most prevalent bacteria that cause sexually transmitted infections in the world.. Most of these infections are asymptomatic and there is a remarkable relationship between CT infections and male infertility. Therefore, the present study is established to determine the effect of Chlamydia trachomatis infection on seminal fluid quality in males suffering from infertility compared with fertile males.. Semen samples were collected from 63 infertile male and 13 fertile male as control group, attending the clinical laboratory for routine seminal fluid analysis. Seminal fluid was analyzed according to World Health Organization guidelines, the whole genomic DNA was extracted for molecular study. Real time PCR technique was used to specifically detect the presence of CT DNA in semen samples.
- trachomatis was demonstrated in the semen samples of 11 (17.4%) infertile male and all control samples were negative. Infertile, infected males had semen samples that showed statistically significant differences in the mean of total sperm count, motility and morphology as compared with fertile uninfected control samples. These differences indicate that chlamydial infection of genital tract could negatively influence the quality of seminal fluid.