Estimate Antimicrobial activity and Anti-biofilm formation of bark Cinnamomum zeylanicum on Klebsiella pneumoniae isolated from Urinary Tract Infections

  • Enass Ghassan Sweedan Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq

Abstract

Five isolates (25%) of Klebsiella pneumoniae were isolated from urine samples. In addition also isolated bacteria were (10) 50% Escherichia coli, while (3)15% Proteus spp., (2)10% Pseudomonas aeruginosa. The ethanolic extract of Cinnamomum zeylanicum bark were tested against Klebsiella pneumoniae by using the well agar diffusion test, the alcoholic bark extract from (200 -12.5) mg/ml possessed antimicrobial activity against tested microorganism. At 200 mg/ml, and 100 mg/ml concentrations was diameter of inhibition zone rang from (18-26mm), (14-16mm) respectively, and these results compared to antibiotics Norfloxacin(10µg) inhibition zone (24-30mm), and Cefotaxim (10 µg) (26-27mm) as control. Antibiotics sensetivity were used ten antibiotics with disc diffusion method against K. pneumoniae, they were sensitive to Cefotaxime, Imipenem /Cilactin,Sparfloxacin, and  Norfloxacin antibiotics only, and were resisted to the others antibiotics. Minimum inhibitory concentration was tested for plant extract, and was ranged versus Klebsiella pneumoniae (12.5-25) mg/ml. It could be concluded that alcoholic extract of Cinnamomum zeylanicum had a good antimicrobial effects and anti-biofilm on Klebsiella pneumoniae with significant difference (p< 0.05). This caused a decrease in the biofilm of the tested bacteria. Hence, it may be used instead of antibiotics to treat UTIs caused by bacteria used in current study.

Published
Sep 25, 2018
How to Cite
SWEEDAN, Enass Ghassan. Estimate Antimicrobial activity and Anti-biofilm formation of bark Cinnamomum zeylanicum on Klebsiella pneumoniae isolated from Urinary Tract Infections. Iraqi Journal of Science, [S.l.], p. 1560-1566, sep. 2018. ISSN 2312-1637. Available at: <http://scbaghdad.edu.iq/eijs/index.php/eijs/article/view/503>. Date accessed: 15 dec. 2018.
Section
Biology