The inhibitory effect of Trichoderma harzianum CA-07 crude extract against Trichophyton mentagrophyte and Microsporium canis

  • Surra Rasan Aasi Department of Biology, University of Baghdad, College of Science, Baghdad, Iraq
  • Alaa Mohsen Al-Aaraji Department of Biology, University of Baghdad, College of Science, Baghdad, Iraq

Abstract

The study aimed to identify Trichoderma harzianum isolates morphologically and by using PCR teqnique and evaluate the antifungal activity of T. harzianum CA-07  crude extract against Trichophyton mentagrophytes and Microsporium canis from patients with dermatophytosis. T.harzianum isolates were collected from the soil of Baghdad University gardens and they were identified depending of morphological features on plate and microscopic examination. The genomic DNA of T.harzianum isolate was extracted at a final concentration of (400-600) μg / 2-3 g of wet mycelium and at a purity of 1.6-1.8and DNA sample was amplified with each of universal primers (ITS-1& ITS-4) to be used for detection of Trichoderma species. The crude extract was extracted from T.harzianum strain CA-07 by ethyl acetate with finalyield of 4.8gm., then different crude extract concentrations (0.5, 1, 2 and 4 mg/ml) were used against the clinical pathogenic fungi (T.mentagrophyte and M.canis) using agar well diffusion method. The results of identification of Trichoderma harzianum isolates by using PCR confirmed that the isolate was T.harzianum strain CA-07,and their crude extract exhibited significantly high antifungal activity against T. mentagrophyte and M. canis with high growth inhibition zones(14, 12mm) respectively at the lower concentration (0.5mg/ml)of crude extract.

Published
Aug 30, 2018
How to Cite
AASI, Surra Rasan; AL-AARAJI, Alaa Mohsen. The inhibitory effect of Trichoderma harzianum CA-07 crude extract against Trichophyton mentagrophyte and Microsporium canis. Iraqi Journal of Science, [S.l.], p. 1387-1395, aug. 2018. ISSN 2312-1637. Available at: <http://scbaghdad.edu.iq/eijs/index.php/eijs/article/view/469>. Date accessed: 20 sep. 2018.
Section
Biology