A Molecular Study of Toxic Shock Syndrome Toxin gene (tsst-1) in β-lactam Resistant Staphylococcus aureus Clinical Isolates

  • Nariman Nidhal Omar Department of Biotechnology, College of Science, University of Baghdad, Baghdad, Iraq
  • Rana Kadhim Mohammed Department of Biotechnology, College of Science, University of Baghdad, Baghdad, Iraq
Keywords: tsst-1 gene, S. aureus, β-lactam resistance, Multiple Drug Resistance, Clinical isolates

Abstract

Three hundred and sixty different samples were collected from different sources, including wound, burn, nasal, and oral swabs from several hospitals in Baghdad. A number of 150 (53%) Staphylococcus aureus samples were isolated and identified among a total of 283 samples. Then, the spread of the Toxic Shock Syndrome Toxin-1 gene (tsst-1) was investigated in β-lactamase resistant S. aureus. According to the source of samples, the distribution of S. aureus  isolates was found to be significantly higher (p < 0.01) in wound samples as compared to other sources. According to the age, a highly significant  distribution (p < 0.01) was recorded in the age group of 15-30 years, whereas gender comparison showed no statistically significant differences. All the isolates were subjected to susceptibility test against eight β-Lactam antibiotics by using the disc diffusion method. The antimicrobial susceptibility test showed that S. aureus had maximum resistance percentage to Carbenicillin (97.3%), while the lowest resistance rate was against Meropenem, with a sensitivity rate of up to 82%. In addition, 144 (96%) out of the 150 S. aureus isolates have multiple drug resistance (MDR). All the isolates were subjected to polymerase chain reaction to amplify tsst-1 toxin gene. A number of 70 isolates (46.7%) were found to be positive for tsst-1 gene. The results showed no significant correlation between tsst-1 gene with the individual antibiotic resistance and the multi-drug resistance patterns of the isolates (p = 0.226).

Published
2021-03-30
How to Cite
Nariman Nidhal Omar, & Rana Kadhim Mohammed. (2021). A Molecular Study of Toxic Shock Syndrome Toxin gene (tsst-1) in β-lactam Resistant Staphylococcus aureus Clinical Isolates. Iraqi Journal of Science, 62(3), 825-837. https://doi.org/10.24996/ijs.2021.62.3.13
Section
Biotechnology