Assessment of genetic diversity among wheat selectedegenotypese and local varieties for saltetolerance by usingeRAPD and ISSR analysis

  • Duha Mysire Majeed Biotechnology Research Center, Al-Nahrine University, Baghdad, Iraq.
  • Eman Noaman Ismail Biotechnology Research Center, Al-Nahrine University, Baghdad, Iraq.
  • Ibrahim Ismail Al-Mishhadani Biotechnology Research Center, Al-Nahrine University, Baghdad, Iraq.
  • Noor Mohammed Sakran College of Biotechnology, Al-Nahrain University, Baghdad, Iraq.

Abstract

Three genotypes  of wheat developede for saltetoleranceethroughe plant breeding program and two local cultivars were screened for genetic variation under salinity conditions through RAPD and ISSR markers. Eight selected primers (OP1-06, OPE-16, OPN-07, OPO-17, OPD-20, OPL-05, OPI-01 and OPJ-13) were used in randomeamplifiede polymorphic eDNA (RAPD-PCR reaction) and three selected primers (UBC 809, UBC 810 and UBC 811) were used in ISSR markers. According to the results of the amplification and ISSR markers, the genetic distance and dendogram illustratedegeneticefingerprint and relationshipsebetweeneselected genotypes and local cultivars were determinant. Results revealed that there are genetics differences between the selected genotypes and the local cultivars in some specific segment at different size (bp) with all primers which used in this study, except the result of the primer (OPE-16) showed that there are no bands appeared in all selected genotypes and local cultivars. Other results of RAPD markers showed that there are differences among the selected genotypes in their banding patterns only with primers (OP1-06, OPN-07 and OPO-17) at different size. The results of ISSR markers showed differences that there are also differences between the selected genotypes and local cultivars in specific segment with the three primers which used, the selected genotypes were similar in banding patterns with UBC 809 and UBC 811primers. Genetically, the results showed that all the selected genotypes and local cultivars differed in their genetic distance, variations among the selected genotypes in their genetic distance. In conclusion the selected genotypes (salt tolerant) genetically differed from the local cultivars (salt sensitive).

Published
Mar 4, 2018
How to Cite
MAJEED, Duha Mysire et al. Assessment of genetic diversity among wheat selectedegenotypese and local varieties for saltetolerance by usingeRAPD and ISSR analysis. Iraqi Journal of Science, [S.l.], p. 278-286, mar. 2018. ISSN 2312-1637. Available at: <http://scbaghdad.edu.iq/eijs/index.php/eijs/article/view/213>. Date accessed: 22 june 2018.
Section
Biotechnology