Immunocytochemical assessment of FKBP51 and Glucocorticoid receptor localization in asthmatic patients

  • Sura F. Alsaffar Department of Biology, college of science, Baghdad University , Baghdad, Iraq.
  • Jabbar H. Yenzeel Department of Biology, college of science, Baghdad University , Baghdad, Iraq.
  • Haider Faisal Ghazi Department of Microbiology, College of medicine, Al-nahrain University, Baghdad, Iraq
Keywords: Asthma, FKBP51, Glucocorticoid Receptor, ACTH, Cortisol

Abstract

Asthma is a chronic inflammatory disease affecting 5% of the world population. FKBP51 is an important immunophilin modular protein of the glucocorticoid receptor (GC).

The aim of the present study was to evaluate the levels and immunocytochemical distribution of FKBP51 and GR in lymphocyte cells of asthmatic patients, by use of immunocytochemistry method, and to assesslevels ofstress hormones (cortisol and ACTH) by radioimmuniassay (RIA).

The results showed significantly increased nuclear localization and decreasedcytoplasmic distribution of FKBP51, while they showed a significant increase in nuclear localization and a non-significant decrease in cytoplasmic distribution of GRin asthmatic patients(P<0.05).

Cortisol and ACTH levels were also measured and showedinsignificant increases(P<0.05)in steroid treated (338.85 ±139.5 mMol/L, 35.05±3.77 ng/ml, respectively)and non steroid treated asthmatics(280.5 ± 74.6 mMol/L, 32.0±6.43 ng/ml, respectively)as compared with the control group (234.33±29.13 mMol/L, 29.0±7.02 ng/ml, respectively).

Author Biography

Jabbar H. Yenzeel, Department of Biology, college of science, Baghdad University , Baghdad, Iraq.

Biology department

Published
2019-12-29
How to Cite
AlsaffarS. F., YenzeelJ. H., & GhaziH. F. (2019). Immunocytochemical assessment of FKBP51 and Glucocorticoid receptor localization in asthmatic patients. Iraqi Journal of Science, 60(12), 2609-2617. Retrieved from http://scbaghdad.edu.iq/eijs/index.php/eijs/article/view/1372
Section
Biology